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MultiCell Technologies
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RainDance Technologies
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METTLER TOLEDO
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FLUIGENT Inc
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Encapsula NanoSciences LLC
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BioMimetic Therapeutics
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Macrogen
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Image Search Results
Journal: STAR Protocols
Article Title: CloneSeq - Single-cell clonal 3D culture and analysis protocol
doi: 10.1016/j.xpro.2021.100794
Figure Lengend Snippet: Design of microfluidic devices (A) Device I: single cell encapsulation device for PEGDT-MALDEX hydrogel culturing. (B) Device II: CloneSeq device for single-clone transcriptome barcoding in drops. The numbers indicate the inlets: (1) carrier oil inlet, (2) PEGDT mix, (3) MALDEX mix with cells, (4) outlet for collection, (5) single clone suspension, (6) RT lysis mix, (7) barcoding hydrogel bead.
Article Snippet: For the use of A-i-O software, check the manual of
Techniques: Lysis
Journal: STAR Protocols
Article Title: CloneSeq - Single-cell clonal 3D culture and analysis protocol
doi: 10.1016/j.xpro.2021.100794
Figure Lengend Snippet: Microfluidic setup for single cell encapsulation in PEGDT-MALDEX hydrogel sphere (A) Pressure controller. Use 2000 mbar pump for carrier oil inlet, 1000 mbar pump for PEGDT and MALDEX inlets. (B) Pressurized reservoir. Use 1.5 mL and 2 mL Safe-Lock tubes for PEGDT and MALDEX mix, respectively and use a 15 mL tube for 2% HFE-7500 oil. (C) Cell mixer. The yellow square shows a zoom in on the MALDEX mix. The tube is surrounded by an ice stand, and the cell mixer is working outside of the tube towards the bottom of the tube, to make the magnetic stirring bar agitate the cells and prevent sedimentation. (D) Flow sensor. Use Flow unit size M for all three inlets. (E) Microfluidic device for single cell encapsulation in PEGDT-MALDEX hydrogel sphere. A zoom in on the design, indicating the position of each inlet: (1) 2% HFE-7500 oil, (2) PEGDT mix, (3) MALDEX mix and (4) outlet for collection. (F) Collection tube. Use a 2 mL tube, placed in a cooling block. (G) Control software for the microscope, zoom in on the droplet-generation junction. (H) A-i-O software for flow control.
Article Snippet: For the use of A-i-O software, check the manual of
Techniques: Sedimentation, Blocking Assay, Software, Microscopy
Journal: STAR Protocols
Article Title: CloneSeq - Single-cell clonal 3D culture and analysis protocol
doi: 10.1016/j.xpro.2021.100794
Figure Lengend Snippet: Single cell encapsulation in PEGCT-MALDEX hydrogel sphere Red arrows indicate boundary where PEGDT and MALDEX meet. Scale bar: 50 μm.
Article Snippet: For the use of A-i-O software, check the manual of
Techniques:
Journal: STAR Protocols
Article Title: CloneSeq - Single-cell clonal 3D culture and analysis protocol
doi: 10.1016/j.xpro.2021.100794
Figure Lengend Snippet: Microscope photo of ESC in PEGDT-MALDEX hydrogel after encapsulation The big transparent spheres are PEGDT-MALDEX hydrogel spheres. They are about the size of 70 μm diameter. Red arrows indicate single cells in hydrogel spheres. Yellow arrows indicate oil drops. Scale bar: 50 μm.
Article Snippet: For the use of A-i-O software, check the manual of
Techniques: Microscopy
Journal: STAR Protocols
Article Title: CloneSeq - Single-cell clonal 3D culture and analysis protocol
doi: 10.1016/j.xpro.2021.100794
Figure Lengend Snippet: ESCs in PEGDT-MALDEX hydrogel Left picture shows the cells after encapsulation into hydrogel, right picture shows clones developed after 3 days of culture. The round-shaped cells are clones well defined in hydrogels, and cells with dendrites are cells that escaped from the hydrogel and grew on the tissue culture plate. Scale bars: 50 μm.
Article Snippet: For the use of A-i-O software, check the manual of
Techniques: Clone Assay